How to perform an Oxidase test in microbiology for gonorrhea and non fermenters

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2023-3-21 18:21

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Oxidase Test is a microbiology procedure to detect cytochrome oxidase activity in bacteria. Cytochrome containing organisms produce an intracellular oxidase enzyme. This oxidase enzyme catalyzes the oxidation of cytochrome c. Organisms which contain cytochrome c as part of their respiratory chain are oxidase- positive and turn the reagent blue/purple. Organisms lacking cytochrome c as part of their respiratory chain do not oxidize the reagent, leaving it colorless within the limits of the test, and are oxidase-negative.

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